General Information: Basal Medium MCDB 201 is specifically formulated for the cultivation of chick fibroblasts in accordance with the formulation by McKeehan and Ham. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed). Also suitable for stem cell and CHO cell growth. The medium is for research use only and not approved for human or animal use or for application in any in vitro diagnostic procedure. Storage of the medium and supplements Please note that the basal medium has to be stored at 4°C up to 8°C but never should be frozen. The supplement has to be stored at -20°C. Protect from light for a longer time period. Preparing and Stability: Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol excess before opening the cap of the tube. After adding the supplement mixture to the basal medium slew carefully to get a homogenous medium. Please be careful not to touch the tube or bottle interior threads with your fingers. Please avoid long term exposure to light because some components are light sensitive. Also do not warm up the medium over 37°C. All this protects your medium against contamination or degradation of essential components